https://nova.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Establishment and characterization of oviductal organoids from farm and companion animals(dagger) https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53095 Wed 27 Mar 2024 12:12:14 AEDT ]]> From peptide masses to pregnancy maintenance: a comprehensive proteomic analysis of the early equine embryo secretome, blastocoel fluid, and capsule https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:30545 Wed 15 Dec 2021 16:09:36 AEDT ]]> Conception and early pregnancy in the mare: lipidomics the unexplored frontier https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51207 Thu 24 Aug 2023 15:00:51 AEST ]]> Insights into the NAD+ biosynthesis pathways involved during meiotic maturation and spindle formation in porcine oocytes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51206 Thu 24 Aug 2023 15:00:18 AEST ]]> Supplemental Nicotinic Acid Elevates NAD+ Precursors in the Follicular Fluid of Mares https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51205 Thu 24 Aug 2023 14:59:15 AEST ]]> NAD+, Sirtuins and PARPs: enhancing oocyte developmental competence https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:51204 Thu 24 Aug 2023 14:59:02 AEST ]]> Supplementing media with nad+ precursors enhances the in vitro maturation of porcine oocytes https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39756 Thu 22 Jun 2023 11:39:57 AEST ]]> Characterization of an L-amino acid oxidase in equine spermatozoa https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27633 L-tryptophan > L-tyrosine) were substrates for this enzyme, eliciting the dose- and time-dependent generation of ROS via mechanisms that were enhanced by cell death. This unexpected result was confirmed by analyses of ROS generation in subcellular sperm fractions, which again located a majority of LAAO activity to the sperm head. Equine cryopreservation medium was shown to contain sufficient quantities of aromatic amino acids to activate the LAAO system and generate ROS. The biological significance of this activity was established in an experiment in which physiological concentrations of aromatic amino acids were found to suppress sperm motility but only if dead spermatozoa were present in the same suspension. The combination of aromatic amino acids and nonviable cells was also found to enhance the levels of lipid peroxidation in live spermatozoa. These results suggest the potential significance of LAAO activity in generating the oxidative stress associated with the cryopreservation of equine spermatozoa. It is possible that inhibitors of this enzyme system may facilitate the development of modified cryostorage regimes for clinical validation in vivo.]]> Sat 24 Mar 2018 07:34:05 AEDT ]]> Nicotinic acid supplementation at a supraphysiological dose increases the bioavailability of NAD+ precursors in mares https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:49966 Mon 06 May 2024 15:45:12 AEST ]]>